FIGURE 3.

Flaxseed-oil supplementation alters the monocyte glucose metabolism in women with obesity. Bioenergetics parameters of CD14⁺CD16^– monocytes purified from peripheral blood on day 0 and 28 of each phase were assessed using a Seahorse XF mitochondrial stress test in the presence of glucose. (A) Correlations between BMI and parameters at the first study visit (n = 15). Pearson coefficients (r) and P values are indicated. (B) Supplementation effects on selected bioenergetics parameters (n = 12–15). Data were analyzed using a mixed model, and pairwise comparisons were assessed by difference of least square means with adjusted P values (Tukey-Kramer correction). *P < 0.05. Complete statistical analyses of all bioenergetics parameters are in Table 6 and Supplemental Table 8. (C) Visualization of cell energy phenotypes of unstimulated and 2-hour LPS-stimulated cells. OCR and ECAR measurements represent mean values from 10–15 participants. ECAR, extracellular acidification rate; OCR, oxygen consumption rate; SRC, spare respiratory capacity.