Figure 1.
Inducible pulmonary emphysema leads to reduced respiration in skeletal muscle. EDL muscles from IL13TG (emphysema) and IL13WT (wild-type [WT]) mice were (A) assayed in the isolated contractility platform that demonstrated a reduced fatigue tolerance in IL13TG animals’ muscles (n = 5); (B) assayed in the plate respirometry (Seahorse) platform, which demonstrated a reduced oxygen consumption rate (OCR) in IL13TG animals’ muscles (n = 6); (C) immunostained with antibodies to detect MyHC (myosin heavy chain) isoforms to characterize fiber types, showing similar fiber type distribution in IL13TG and WT mice (n = 6); and (D) processed for EM evaluation, which ruled out any conspicuous structural mitochondrial abnormality (n = 4; data above each panel designate the sex and number of the animal producing the micrography). **P < 0.01 and ***P < 0.001. EDL = extensor digitorum longus; TG = transgenic.