Fig 2. Substitution error profile for bacterial and human datasets.

Results for bacterial datasets are presented with boxplots, those for human are displayed with crosses. A: Results obtained in HAC basecaller mode (unaware of methylation). B: Results obtained in methylation basecaller mode. We focused here on substitution to A and C in reads, the only methylations supported by Guppy (only the C-methylation for human data). For each substitution type, its abundance is given relatively to all substitutions, separating methylated and non-methylated positions.