Figure 2. Receptor pharmacology of hαCGRP and pramlintide at human calcitonin family receptors.

A) CGRP receptor, B) AMY₁ receptor, C) comparing hαCGRP at CGRP and AMY₁ receptors, D) AM₁ receptor, E) CT receptor, F) AM₂ receptor, G) AMY₂ receptor, H) AMY₃ receptor. cAMP production from transiently transfected Cos-7 cells in response to each peptide ligand is shown. Data are mean ± SEM. combined from five independent experiments, each with three technical replicates. Adrenomedullin was used to confirm functionality of the AM receptors (data not shown). Stars on A)-C) indicate the mean peak plasma concentration achieved for each peptide in the assays used in this study; pramlintide (4943 pM, red), CGRP (428 pM, blue) marked on the corresponding concentration-response curve. In A)-H) this is also marked for the relevant peptide with an extra tick on the X axis to show the log molar concentration.