FIGURE 4.
Fluorescent peptides 1131 and 0931 showed specific targeting to NUGC‐4 cells. A, Fixed NUGC‐4 and AGS cells were incubated with fluorescent‐labeled peptides. CG7C was considered a negative control. DAPI, staining of the nucleus (blue); 1131, binding of fluorescent peptides to cells (red, RhoB). Compared with the control, strong fluorescence signals were observed in the 1131 and 0931 groups. Scale bar, 100 μm. B, Competitive inhibition assay. Fixed cells were incubated with mAb (Abcam ab219971) and fluorescent peptides. mAb, binding fluorescence of Abcam ab219971 (green). 1131, a competitive inhibition against mAb. Scale bar, 100 μm. C, Fluorescence intensity histogram of (A). MFI was analyzed using ImageJ software v.1.52. ****P < .0001. D, Fluorescence intensity histogram of (B). MFI was analyzed using ImageJ software v.1.52. *P < .05, **P < .01