FIGURE 1.

CH5126766 inhibits the growth of triple‐negative breast cancer (TNBC) cells with cell cycle arrest and induction of apoptosis. A, Several TNBC cells were treated with the indicated concentrations of CH5126766 for 72 h. The inhibition of cell growth was evaluated using CCK‐8. The cell growth rate of each treatment was calculated by comparisons with DMSO (DM) samples. B, Cell cycle populations treated with CH5126766 were analyzed by flow cytometry. Cell cycle populations at the indicated concentrations of CH5126766 were compared with DMSO samples. MDA‐MB‐231 and SUM159PT cells were treated for 24 h. C, Apoptotic cells were analyzed as the sub‐G₁ population by flow cytometry. Sub‐G₁ populations with the CH5126766 treatment were compared with DMSO samples. MDA‐MB‐231 cells were treated with DMSO (DM) or the indicated concentrations of CH5126766 (C300‐C1000) for 72 h, as well as SUM159PT cells (C500‐C2000) for 24 h. Columns, mean values of triplicate data; bars, SD. *P < .05