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. 2021 Aug 18;112(10):4393–4403. doi: 10.1111/cas.15084

FIGURE 1.

FIGURE 1

Protocol for cfRNA extraction, cDNA synthesis, multiplex PCR, and sequencing. An overview of the cfRNA‐based assay in this study. Whole blood samples were obtained from NSCLC patients. cfRNA extracted from 4‐5 mL of plasma was converted to cDNA by the RT‐RamDA method and subsequently subjected to PCR with primers for the lung cancer‐related fusion genes (7 ALK fusions, 9 RET fusions, 8 ROS1 fusions, 11 NTRK1/2/3 fusions, and 10 FGFR1/2/3 fusions) and MET exon 14 skipping. An NGS library was generated that contained c. 10 K reads on the MiSeq platform. (Image courtesy of Bio‐Rad Laboratories and Illumina)