FIGURE 2.

Alantolactone (ALT) decreases YAP1/TAZ protein levels, nuclear localization, and YAP1/TAZ‐dependent transcriptional activity. A, Left panel: Immunoblot to detect the indicated total and phosphorylated YAP1 and TAZ proteins in H1299‐Luc cells that were treated with/without 10 μmol/L ALT for the indicated times. Actin, loading control. Right panels: Quantification of data in left panel. Data are mean ± SEM of three independent experiments. B, Upper panel: Immunofluorescent detection of nuclear localization of YAP1 in H1299‐Luc cells treated for 3 h with DMSO (ALT (−); control) or 10 μmol/L ALT (ALT (+)). Lower panel: Quantification of data in upper panel. Data are presented as the ratio of nuclear (Nuc) to cytoplasmic (cyto) YAP1 in the indicated individual cultures. Results are representative of three independent experiments. C, Left panel: Quantitative RT‐PCR analysis of YAP1/TAZ and their indicated target genes in H1299‐Luc cells treated for 72 h with either siScr (control) or siYAP1/TAZ. Right panel: Quantitative RT‐PCR analysis of indicated genes in H1299‐Luc cells treated for 10 h with indicated concentrations of ALT. Mock, DMSO. For both panels, data are mean ± SEM of three independent experiments. *P < .05