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. 2021 Aug 6;112(10):4303–4316. doi: 10.1111/cas.15079

FIGURE 3.

FIGURE 3

Alantolactone (ALT) enhances YAP1/TAZ degradation. A, Upper panel: Immunoblot to detect total YAP1 and TAZ proteins in H1299‐Luc cells treated with ALT (10 μmol/L; ALT+) or DMSO (0.1%; ALT−) in combination with 100 μg/mL cycloheximide (CHX) for indicated times. Actin, loading control. Lower panel: Quantification of data in upper panel. Results are mean ± SEM of three independent experiments. B, Quantitative RT‐PCR analysis of YAP1 and TAZ mRNA levels in H1299‐Luc cells treated with 10 μmol/L ALT (ALT (+)) or 0.1% DMSO (ALT (−)) for 1 h. Data are mean ± SEM of three independent experiments. C, Left panel: Immunoblot to detect total YAP1 and TAZ proteins in H1299‐Luc cells treated with ALT (10 μmol/L; ALT+) in combination with MG132 (10 μmol/L) or DMSO (0.1%) for 6 h. GAPDH, loading control. Right panel: Quantification of data in left panel. Results are mean ± SEM of three independent experiments. *P < .05, **P < .01