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. 2021 Aug 23;112(10):4198–4207. doi: 10.1111/cas.15101

FIGURE 4.

FIGURE 4

Tribbles pseudokinase 3 (TRIB3) promoted β‐catenin/Wnt signaling. A, Western blotting of β‐catenin and Wnt3A in HCT116/CT26 cells treated with PBS, transforming growth factor‐β (TGF‐β, 100 ng/mL), TGF‐β (100 ng/mL) combined with GN44028 (20 nmol/L) (T+G), or TGF‐β (100 ng/mL) combined with TRIB3 siRNA (T+Si#1 and T+S#2). B, Colony formation rates of TGF‐β (100 ng/mL) cultured CT26/HCT116 cells treated with PBS or PNU‐74654 (50 μmol/L). C, Tumorigenesis capability of TGF‐β (100 ng/mL) cultured CT26/HCT116 cells treated with PBS or PNU‐74654 (50 μmol/L). D, Relative migrating cell numbers of TGF‐β (100 ng/mL) cultured CT26/HCT116 treated with PBS or PNU‐74654 (50 μmol/L). E, Immunofluorescence staining of Wnt3A in tumor tissues from metastasis (M) and nonmetastasis (N) colorectal cancer patients. Intensity of β‐catenin/Wnt expression was determined in 30 patients. Scale bar, 50 μm. *P < .05. **P < .01