FIGURE 2.

Serum amyloid A1 (SAA1) promotes migration and invasion by colorectal cancer (CRC) cells. A, qRT‐PCR analysis of SAA1 in CRC cell lines. Shown are means of three replications; error bars represent standard errors of the mean (SEMs). B, qRT‐PCR analysis of SAA1 in SW480 cells transfected with a control siRNA (siCtrl) or siRNAs targeting SAA1. Total RNA was extracted 48 h after transfection. Shown are means of three replications; error bars represent SEMs. C, Proliferation of SW480 cells transfected with the indicated siRNAs. Results are means of eight replications; error bars represent SEMs. Results of migration (D) and invasion (E) assays using SW480 cells transfected with the indicated siRNAs. Lower wells contain culture medium supplemented with 10% FBS. Representative results are shown on the left; summarized results are on the right. Results are means of three replications; error bars represent SEMs. F, qRT‐PCR analysis of SAA1 in HCT116 cells infected with the indicated lentiviral vector. Results are means of three replications; error bars represent SEMs. G, Proliferation of HCT116 cells infected with indicated vector. Results are means of eight replications; error bars represent SEMs. Migration (H) and invasion (I) assays using HCT116 cells infected with the indicated vector. Lower wells contain culture medium with 10% FBS. Representative results are shown on the left; summarized results are on the right. Results are means of three replications; error bars represent SEMs. J, Collagen gel invasion assays using HCT116 cells infected with the indicated vector. Results are means of three replications; error bars represent SEMs. *P < .05, **P < .01