FIGURE 6.

Localization of miR‐9‐1 expression in nasopharyngeal carcinoma (NPC) tissue by fluorescence in situ hybridization and correlation with prognosis in patients with NPC. A, The probe for miR‐9‐1 was locked nucleic acid (LNA) modified and labeled with digoxigenin at the 5' end. (a‐c) Strong expression of miR‐9‐1 in NPC. (d‐f) Moderate expression of miR‐9‐1 in NPC. (g‐i) Weak expression of miR‐9‐1 in NPC. Red, miR‐9‐1; Blue, 4′,6‐diamidino‐2‐phenylindole (DAPI) nuclear staining. Scale bars are 10 µm. Original magnification 200×. B, Kaplan‐Meier analysis comparing overall survival, distant metastasis–free survival, and locoregional relapse–free survival between the miR‐9‐1–high‐ and miR‐9‐1–low‐expression groups. Patients with low miR‐9‐1 expression had shorter overall survival and locoregional relapse–free survival. C, Proposed model for miR‐9‐1 modulation of hexokinase 2 (HK2) expression as well as glycolysis‐related tumor growth