FIGURE 6.

Silent information regulator 2 (SIRT2) enhanced glucose 6‐phosphate dehydrogenase (G6PD) activity by deacetylation in clear cell renal cell carcinoma. A, G6PD activity was measured in stably transfected ACHN and Caki‐1 cells. **P < .01 vs corresponding controls (unpaired‐sample Student’s t test). B, C, mRNA and total protein expression levels of G6PD in SIRT2‐overexpressing (OE) ACHN cells and SIRT2‐knockdown (KD) Caki‐1 cells were detected by (B) quantitative real‐time PCR and (C) western blot analysis. D, G6PD dimer and monomer expression levels in ACHN SIRT2 OE and Caki‐1 KD cells were analyzed using western blot assays with glutaraldehyde cross‐linking. E, Acetylation level of G6PD and interactions between SIRT2 and G6PD were analyzed by coimmunoprecipitation (Co‐IP) assays in ACHN and Caki‐1 cells. F, G6PD acetylation levels were determined by quantitative Co‐IP analysis in stably transfected ACHN and Caki‐1 cells. Data represent three independent experiments, each carried out in triplicate. IB, immunoblot