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A3A/Y130F outperforms PmCDA1 and hAID in the iSpyMacCas9 based BE3 platform in rice. (a) Comparison of three iSpyMacCas9‐derived CBE_V01 systems at four target sites with A‐rich PAMs in rice protoplasts. The error bars represent standard deviations of three biological replicates. (b) Base editing frequency of iSpyMacCas9‐derived A3A/Y130F‐CBE_V01 in stable rice lines. (c) Genotypes of base edited T0 lines. Note the edited positions are indicated by asterisks.
