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. 2021 Jun 5;19(10):2082–2096. doi: 10.1111/pbi.13638

Figure 3.

Figure 3

PpTPS3 catalyses linalool synthesis both in vitro and in vivo. (a) Enzymatic activity assay of PpTPS3 protein towards geranyl pyrophosphate (GPP) as substrate in vitro. (b) Schematic diagram for gene transient expression in peach fruits. (c) Transient overexpression of PpTPS3 increases linalool content in peach fruits. Empty SK vector was used as a control. (d) Silencing PpTPS3 by VIGS decreases linalool content in peach fruits. Empty pTRV1 + pTRV2 vector was used as a control. Phytoene desaturase (PDS) gene was used as a reporter gene for VIGS (Figure S3). (e) Overexpressing PpTPS3 induces linalool synthesis in Arabidopsis. tps10 mutant was used as a control. (f) Increased linalool content in tomato fruits after overexpressing PpTPS3. Wild‐type (WT) fruits were used as controls. (g) Subcellular localization of PpTPS3. Bars = 20 µm. Data are presented as mean ± standard error from three independent biological replicates. Significant differences are indicated with asterisks above the bars (*, P < 0.05; **, P < 0.01; and ***, P < 0.001). N.D., not detected.