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. 2021 Jun 5;19(10):2082–2096. doi: 10.1111/pbi.13638

Figure 4.

Figure 4

PpbHLH1 activates PpTPS3 and binds to its promoter. (a) Regulatory effects of transcription factors on the promoter of PpTPS3. Means and standard errors were calculated from six replicates. (b) Yeast one‐hybrid analysis of PpbHLH1 binding to the PpTPS3 promoter. Autoactivation was tested on SD‐Ura in the presence of AbA. AD‐empty and pAbAi‐PpTPS3 were used as negative controls. (c) G‐box (CACGTG) and E‐boxes (CACATG) of bHLH protein‐binding sites in the PpTPS3 promoter. (d) EMSA of 3’ boitin‐labelled dsDNA probes with the PpbHLH1‐binding protein. Presence or absence of specific probes is marked by symbol + or ‐. The mutated nucleotides in probe are indicated in red lowercase letters. (e) Subcellular localization of PpbHLH1 in Nicotiana benthamiana leaves. GFP, GFP channel; nucleus–RFP, transgenic tobacco plants with red fluorescence in the nucleus; merge, merged image of the GFP and nucleus–RFP channels; bright‐field, light microscopy image; bars = 50 µm.