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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: ACS Appl Bio Mater. 2020 Aug 4;3(9):6263–6272. doi: 10.1021/acsabm.0c00761

Figure 4. Preparation, characterization, and stability assessment of immunoplexes (IPs).

Figure 4.

A) Schematic representation of enveloping Ac-PEI/GFP polyplexes with various wt.% of PEMA to form IPs. B, C) Hydrodynamic size and zeta potential of IPs, respectively. Agarose gel electrophoresis to assess the stability of Ac-PEI/GFP polyplexes following PEMA enveloping at N/P 30 in the absence (D) or presence (E) of physiologically-relevant concentration serum (55% v/v). IPs are stable after enveloping with PEMA and in presence of serum as no DNA band is observed. PEMA (poly(ethylene-alt-maleic acid)). n=3 for each experiment. Statistical differences between groups were determined by performing a one-way ANOVA and Tukey’s post hoc test (p < 0.05). All bars in panel B are significantly different from each other. Error bars represent SD.