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. 2021 Sep 17;12:717483. doi: 10.3389/fimmu.2021.717483

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Copyright © 2021 Zhang, Ding, Wang, Yin, Zhang and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CXCL5 induces the EMT process to promote trophoblast migration and invasion. HTR-8 cells were treated with 0, 50, and 100 ng/ml of rhCXCL5 for 48 h. (A, B) Scratch wound healing assay and quantitation were performed to examine trophoblast migration ability. (C, D) Matrigel invasion assay and quantitation were used to detect trophoblast invasiveness ability. (E, F) Western blotting and quantitation were determined to examine the expression levels of E-cadherin, N-cadherin, and vimentin. Representative images of migratory and invasive cells are presented. Data are presented as the mean ± SD. Results are reported as fold change compared with the control group (*p < 0.05, **p < 0.01). rhCXCL5, recombinant CXCL5; EMT, epithelial-to-mesenchymal transition; E-cad, E-cadherin; N-cad, N-cadherin; Vim, vimentin.