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. 2021 Sep 17;12:717483. doi: 10.3389/fimmu.2021.717483

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Copyright © 2021 Zhang, Ding, Wang, Yin, Zhang and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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E, P, HCG, and DSCs regulate CXCL5/CXCR2 expression of trophoblast cells. HTR-8 cells were treated with E, P, and HCG at the indicated concentrations for 48 h. (A, B) RT-PCR was used to detect the expression levels of CXCL5 and CXCR2. (C) A co-culture system of HTR-8 cells and DSCs was taken for 48 h. (D, E) RT-PCR was used to investigate the impact of DSCs on CXCL5 and CXCR2 expression of trophoblast cells. Graphs show the mean ± SD. Results are reported as fold change compared with the control group (*p < 0.05, **p < 0.01, ns, no significant difference, p > 0.05). E, estrogen; P, progesterone; HCG, human chorionic gonadotropin; DSCs, decidual stromal cells; RT-PCR, quantitative real-time PCR.