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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Cell. 2019 Jan 24;176(3):625–635.e14. doi: 10.1016/j.cell.2018.12.030

Figure 3. SFL Interacts with Ribosomes.

Figure 3.

(A) Ribosomes in the lysates of 293T cells transiently expressing SFL-Myc, SFLS-Myc, or GFP-Myc were pelleted and analyzed by western blotting. RPS16 is an endogenous ribosomal protein.

(B) HeLa cells were transfected with a control siRNA or siRNAs targeting SFL. Ribosomes were pelleted and analyzed by western blotting.

(C) SFL-Myc (left) or SFLS-Myc (right) was transiently expressed in 293T cells with GFP-Myc. The cell lysates were subjected to sucrose gradient centrifugation, followed by polysome profiling analysis. Protein levels in each fraction were analyzed by western blotting.

(D and E) Interactions of SFL-Myc with Flag-tagged ribosome large-subunit (D) and small-subunit (E) proteins in 293T cells.

See also Figure S3.