(A) Positions of uL5, eS31, and mRNA entry tunnel in the nonrotated state (left) and rotated state (right) of the ribosome. The structures are adapted from a previously published paper (Svidritskiy et al., 2014).
(B) A working model for SFL inhibition of −1PRF. When the translating ribosome is in a noncanonical rotated state during the process of −1PRF, SFL stably binds to the ribosome, causing ribosome stalling. The eRF1-eRF3 complex is recruited to rescue the stalled ribosome, leading to the production of PMT product.
See also Figure S7.