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. Author manuscript; available in PMC: 2022 Jan 27.
Published in final edited form as: Sci Signal. 2021 Jul 27;14(693):eabg3555. doi: 10.1126/scisignal.abg3555

Figure 6. Excessive LRRK2 kinase activity inhibits AP2M1 uncoating.

Figure 6.

(A) Protein profile of the total homogenates and CCVs purified from mouse brains after SDS-PAGE and Coomassie blue staining. (B and C) Characterization of purified CCVs by immunoblotting with antibodies to clathrin heavy and light chains (B) and with antibodies to CCV-enriched proteins as indicated (C). (D and E) Immunoblotting analysis of uncoated AP2M1 from CCVs. Purified CCVs from mouse brains were incubated with purified GST-LRRK2 GS or DA in the presence of an ATP-regenerating system including ATP and Hsc70. Images was quantified by ImageJ software. Data are the means ± SEM, n=5 independent experiments, with CCVs purified from brain tissue homogenates from 10 mice in each experiment. **P < 0.01 by one-way ANOVA followed by a Tukey’s post hoc test.