Figure 10.

FISH reveals increased adhesion of environmental bacteria on healthy corneas of TRPV1^−/− mice compared to WT and TRPA1^−/− mice. A. Quantification of viable bacteria detected on healthy corneas of WT (none detected), TRPA1^−/− (1 ± 1 bacteria/field of view) and TRPV1^−/− (6 ± 4 bacteria/field of view) by FISH labeling using a universal 16S rRNA gene probe. Data are expressed as the mean ± SEM (area of 1600 μm by 1600 μm). ** P < 0.005 (Two-way ANOVA), WT vs. TRPA1−/− was not significant (Two-Way ANOVA). B. Representative confocal images of WT, TRPA1^−/− and TRPV1^−/− corneas (red) after FISH labeling of any environmental bacteria detected (green, arrows). Scale bar = 50 μm.