Figure 6.

TRPA1^−/−/TRPV1^−/− corneas show reduced CD45+ cell infiltration after bacterial challenge compared to WT mice. A. Quantification of CD45+ cells in healthy (untreated) corneas of control and TRPA1^−/−/TRPV1^−/− mice at baseline and at 4 h after P. aeruginosa challenge. A significant increase in CD45+ cells was observed after P. aeruginosa inoculation in control mice that was not observed in TRPA1^−/−/TRPV1^−/− mice. * P < 0.05, ns = Not Significant (One-way ANOVA with Tukey’s multiple comparisons test). B. Representative images (maximum intensity projections) of CD45+ cells in the central murine cornea in each condition. Scale bar = 50 μm.