Figure 9.
TRPA1−/− corneas show significantly greater bacterial adhesion compared to WT in vivo. A. Quantification of bacterial adhesion to healthy and blotted murine corneas after inoculation of ~1×1011 CFU/mL every hour for 4 h comparing TRPA1−/− mice to WT. Healthy TRPA1−/− corneas showed ~3.8-fold greater adhesion than WT, and blotted TRPA1−/− corneas showed a ~5.1-fold increase over WT. * P < 0.05 (Two-way ANOVA). B. Representative images of P. aeruginosa (green) adhering to the murine cornea (red) in each condition. Scale bar = 50 μm.