Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens) | SMN1 | GenBank | HGNC:HGNC:11,117 | |
| Gene (Homo sapiens) | SMN2 | GenBank | HGNC:HGNC:11,118 | |
| miR (Homo sapiens) | hsa-miR-181a-5p | miRBase | MIMAT0000256 | |
| miR (Homo sapiens) | hsa-miR-324-5p | miRBase | MIMAT0000761 | |
| miR (Homo sapiens) | hsa-miR-451a | miRBase | MIMAT0001631 | |
| Strain, strain background (Mus musculus) | SMNΔ7 miceFVB.Cg-Grm7Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4,299Ahmb/J | Jackson Laboratory | Stock number: 005025 | Hum Mol Genet 14(6):845–57, 2005 |
| Genetic reagent (Homo sapiens) | miRCURY LNA microRNA mimic: hsa-miR-181a-5p, hsa-miR-324-5p, hsa-miR-451a | Exiqon | 50–100–200 nM | |
| Genetic reagent (Homo sapiens) | miRCURY LNA microRNA antogomiR: hsa-miR-181a-5p, hsa-miR-324-5p, hsa-miR-451a | Exiqon | 0.1 nM | |
| Cell line (Homo sapiens) | Primary myoblasts | Italian Telethon Network of Genetic Biobanks | 6756, 6760, 6762, 6816, 7147, 8823, 8655, 8537 |
|
| Biological sample (Homo sapiens) | Muscular biopsies | Italian Telethon Network of Genetic Biobanks | 10370, 10351, 8023, 4688, 10543, 10583, 7669, 5944, 5824, 5717, 6760, 6438, 6082, 5379, 7689, 5842, 9814 |
|
| Sequence-based reagent | See Supplementary file 3 | IDT (Integrated DNA Technologies) | ||
| Commercial assay or kit | TruSeq Small RNA Sample Preparation kit | Illumina | TruSeq Small RNA Library Prep Kit – RS-200-0024 |
|
| Commercial assay or kit | miRCURY RNA Isolation Kit – Biofluids | Exiqon | ||
| Commercial assay or kit | Universal cDNA synthesis kit II | Exiqon | ||
| Commercial assay or kit | Pick-&-Mix miRNA PCR Panel 96-well | Exiqon | ||
| Commercial assay or kit | E.Z.N.A PX Blood RNA Kit | Omega bio-tek | SKU: R1057-01 | |
| Commercial assay or kit | High Capacity cDNA Reverse Transcription Kit | Thermo Fisher Scientific | Catalog number: 4368814 | |
| Software, algorithm | Illumina Genome Analyzer | Illumina | ||
| Software, algorithm | RealTime StatMinerVersion 4.1 | |||
| Software, algorithm | Statgraphics Centurion XV software | StatPoint Inc | ||
| Software, algorithm | SPSS 18.0 software | SPSS | RRID:SCR_002865 |