(
A) Representative confocal images for the data at 0 hr in
Figure 4B. Arrowheads in the inset indicate
Akita puncta colocalizing with SEC24C. (
B) Bar graph showing the % of
Akita-sfGFP puncta colocalizing with mCherry-SEC24C or mCherry-SEC24A puncta for the data in
Figure 4B at 0 hr. (
C)
Akita colocalized with SEC24C and SEC23A. Cells expressing
Akita-sfGFP and mCherry-SEC24C or mRuby-SEC23A were analyzed by confocal microscopy. Arrowheads in the inset indicate
Akita puncta colocalizing with SEC24C (top) or SEC23A (bottom). (
D) Bar graph showing the % of
Akita-sfGFP puncta colocalizing with mCherry-SEC24C or mRuby-SEC23A puncta for the data shown in (
C). Scale bars in (
A) and (
C), 10 µm. Error bars in (
B) and (
D) represent SEM; n = 3 independent experiments. Approximately 20–30 cells/experiment were analyzed. NS: not significant (p≥0.05); *p<0.05, Student’s unpaired
t-test.