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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Biochem J. 2008 Jan 1;409(1):311–320. doi: 10.1042/BJ20070374

Figure 1. Kinetics of [14C]butyrate uptake and effect of different agents in BCS-TC2 cells.

Figure 1

Measurement of butyrate transport at 10–150 mM butyrate. (A) Butyrate transport at 37°C (black circles) and 4°C (white circles). Discontinuous lines represent the non-linear regression of data at 37°C showing a hyperbolic and a linear component. (B) Effect of structural analogues, inhibitors and ions on butyrate uptake. Cells were pre-incubated at the indicated agent concentration or in sodium-free buffer (–Na+) for 15 min before measuring uptake in the presence of 20 mM butyrate. Results shown represent specific uptake expressed as a percentage of the control uptake; results are the means± S.D. for four independent experiments with triplicate samples (**P <0.01).