Figure 8. MCT1 silencing using specific siRNAs and influence on [¹⁴C]butyrate uptake in BCS-TC2 cells.

(A) BCS-TC2 cells were transfected with 500 nM scrambled siRNA (S) or with 250 or 500 nM siRNA 1 (si1) or 2 (si2) respectively. At 72 h after transfection, cells were homogenized and analysed by Western blotting using MCT1- or vinculin (Vin)-specific antibodies. Representative blots are shown. (B) Densitometric analysis of MCT1 levels using vinculin expression for normalization. Results correspond to mean values ± S.D. for four independent experiments. (C) Specific transport at 40 μM butyrate was analysed in parallel untransfected and transfected cultures. Results correspond to mean values ± S.D. for three independent experiments using triplicate samples.