Table 2.
Kinetic parameters of the high-affinity/low-capacity [14C]butyrate transport and phosphatase alkaline activity in human colon adenocarcinoma cells
| Cell line | Vmax × 104 (nmol · min−1 · μg−1) | Km (μM) | Alkaline phosphatase activity (m-units/mg)* |
|---|---|---|---|
| BCS-TC2 | 0.71 ± 0.04 | 17.9 ± 4.0 | 27.3 ± 1.3 |
| BCS-TC2.BR2 | 0.99 ± 0.06 | 14.0 ± 3.6 | 65.8 ± 2.6 |
| HT-29 | 2.93 ± 0.11 | 13.9 ± 2.5 | 6.8 ± 0.8 |
| Caco-2 | 4.70 ± 0.18 | 23.0 ± 3.6 | 162.0 ± 2.7 |
| FHC | 3.42 ± 0.22 | 14.5 ± 4.9 | 466.0 ± 18.1 |
*
Alkaline phosphatase activity was determined under the same conditions as butyrate uptake.