TABLE 1.
Restriction analysis of cdt PCR products
| Strain | Primer pair and enzyme(s)a
|
||||||
|---|---|---|---|---|---|---|---|
| VAT2-WMI1
|
VAT2–LPF-D
|
GNW-WMI1, EcoRI | IVH-WMI1, EcoRI | ||||
| EcoRI | StuI | AluI | BglII | SspI | |||
| C. jejuni | |||||||
| 81-176 | + | + | + | + | + | + | + |
| 84-142 | + | + | + | + | + | + | + |
| 85-452 | + | + | + | + | + | + | + |
| 79-193 | + | + | + | + | + | + | + |
| 85-360 | + | + | + | + | + | + | + |
| G13 | + | + | + | + | + | + | + |
| D133 | + | + | + | − | + | + | + |
| 79-101 | + | + | + | + | + | + | + |
| Lior19 | + | + | + | + | + | + | + |
| AED973 | + | + | + | + | + | + | + |
| AED974 | NP | NP | NP | − | + | NP | NP |
| AEB9710 | + | + | + | + | + | + | + |
| 84-19 | NP | NP | NP | − | + | NP | NP |
| C. coli | |||||||
| 43473 | − | − | + | + | ND | − | − |
| D730 | − | − | + | + | ND | − | − |
| D2593 | − | − | + | + | ND | − | − |
| 78-64 | − | − | + | + | ND | − | − |
| D2594 | − | − | + | + | ND | − | − |
| D115 | − | − | + | + | ND | − | − |
| D126 | − | − | + | + | ND | − | − |
| AEB971 | − | − | + | + | ND | − | − |
| AEB979 | − | − | + | + | ND | − | − |
| AEB9713b | − | − | + | + | ND | − | − |
| AED9715 | − | − | + | + | ND | − | − |
a
Symbols: +, PCR product was cut by the indicated restriction endonuclease; −, PCR product was not cut by the indicated restriction endonuclease. NP, no product produced; ND, not determined.