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DiAcSpm regulates proliferation by targeted upregulating cystathionine β synthase (CBS). (a) The CCK-8 assay showed that the growth-promoting effect of DiAcSpm was abolished in SW480 and Caco-2 cells treated with CBS siRNA (∗P < 0.05 DiAcSpm+/CBS siRNA− vs. DiAcSpm−/CBS siRNA−). (b) Western blotting showed that the effects of DiAcSpm on cyclin D1 and cyclin E upregulation were partially abolished in SW480 and Caco-2 cells treated with CBS siRNA.
