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. 2021 May 11;56(10):2336–2354. doi: 10.1038/s41409-021-01307-9

Fig. 1. Significant reduction of alloreactive T cells both in the CD4+ and CD8+ T-cell compartment through CD276 depletion from CD45RA-depleted responder cells in haplo-MLC.

Fig. 1

After stimulation with irradiated haploidentical PBMCs, CD276+ T cells were removed on day 4, and remaining responder cells were tested by flow cytometry. a Alloreactive marker expression among CD4+ T cells and CD8+ T cells at day 11 (n = 5). b After depletion of CD276+ cells from CD45RA-depleted MLCs at day 4, remaining responder cells were CFSE labeled and analyzed in haplo-MLC using irradiated haploidentical stimulator cells or autologous cells for control. Proliferation was measured at day 8 (n = 3). All experiments were repeated twice by using the identical donors with consistent results. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by one-way ANOVA followed by Tukey’s multiple comparison test.