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. 2021 May 11;56(10):2336–2354. doi: 10.1038/s41409-021-01307-9

Fig. 4. CD45RA/CD276 depletion reduces IFN-γ and TNF-α secretion and activation status of peripheral T cells in DR4-mismatched transplantation.

Fig. 4

a Peripheral blood of DR4 mice was analyzed by flow cytometry for TH1 cytokines secretion, activation markers, and costimulatory/coinhibitory molecules when engraftment of human CD4+ T cells was achieved (days 12–38). b Peripheral IL-17A secreting cells after 4 h stimulation with PMA/Ionomycin were analyzed by flow cytometry when DR4 mice achieved the engraftment of human CD4+ T cells. (n = 6 (bulk CD4+ T cells), n = 5 (memory CD4+ T cells, CD276-depleted memory CD4+ T cells)). Shown are representative data. We performed preliminary experiments at least twice by using the identical donors with consistent results. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by one-way ANOVA followed by Tukey’s multiple comparison test.