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. 2021 May 11;56(10):2336–2354. doi: 10.1038/s41409-021-01307-9

Fig. 5. Scarce T-cell infiltration in skin and colon and less infiltration into liver and lung after transplantation of CD45RA/CD276-depleted grafts.

Fig. 5

a Tissues from NSG-Ab°DR4 mice were fixed in 10% buffered formalin and embedded in paraffin for hematoxylin and eosin (HE) staining and immunohistochemical staining with anti-human CD3. b CD3+ cells were counted in ten representative high-power view fields (×400) under the microscope. c Histopathological GVHD score in lungs and liver of individual mice was evaluated in ten representative high-power view fields (×400) under the microscope. d Resected tissue specimens were disaggregated into single-cell suspensions, and then stained for flow cytometric analysis. The frequencies of human CD4+ T cells were calculated as: %CD4+ T cells/total live cells. (n = 5 (bulk CD4+ T cells), n = 4 (memory CD4+ T cells, CD276-depleted memory CD4+ T cells)). Shown are the representative data. We performed preliminary experiments at least twice by using the identical donors with consistent results. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by one-way ANOVA followed by Tukey’s multiple comparison test.