TABLE 1.
Transforming activity of Vav family proteinsa
| Plasmid | Description | Amount (ng/10-cm plate) | Foci |
|---|---|---|---|
| pNM81 | Wild-type Vav-3 | 1,000 | 0 |
| pNM79 | Vav-3 (Δ1–144) | 1,000 | 0 |
| pNM80 | Vav-3 (Δ1–184) | 1,000 | 0 |
| AatII-pNM81 | Linearized plasmid | 1,000 | 0 |
| AatII-pNM80 | Linearized plasmid | 1,000 | 0 |
| pJC11 | Wild-type Vav | 500 | 0 |
| pKES12 | Vav (Δ1–187) | 100 | TMTCb |
| pXRB138 | Wild-type Vav-2 | 500 | 0 |
| pXRB141 | Vav-2 (Δ1–183) | 300 | TMTC |
| pXEM-LckY505F | LckY505F | 800 | 0 |
| pNM81 + pXEM-LckY505F | Wild-type Vav-3+ LckY505F | 500 + 800 | 0 |
| pJC11 + pXEM-LckY505F | Wild-type Vav+ LckY505F | 500 + 800 | 203 |
| pXRB139 + pXEM-LckY505F | Wild-type Vav-2+ LckY505F | 500 + 800 | 1,344 |
a
Transforming activity was determined using focus formation assays. For plasmid linearization, DNAs were digested with AatII (present in the plasmid backbone), extracted with phenol/chloroform, precipitated thrice with ethanol, and resuspended in sterile water.
b
TMTC, too many to count.