Table 1.
Isoform-specific primers used for qRT-PCR.
| # | Primer name | Amplicon (bp) | Tm (°C) | Primer sequences (5′ → 3′) |
|---|---|---|---|---|
| 1 | f.F3OMT | 165 | 56.4 | CCCTGGTGGTAAGGAAAGGA |
| 2 | r.F3OMT | 55.5 | GAAATGTCGGCACGCTCTTAT | |
| 3 | f.F8OMT | 161 | 56.1 | AGACAATGGTGGGAAGGTGA |
| 4 | r.F8OMT | 56 | TGAAGCCAGCAGCAGAAAAG | |
| 5 | f.CRS | 173 | 56.3 | TTTCAGTAGGCACACGGTCA |
| 6 | r.CRS | 56.3 | AGGGGTACAATACATACCAGCC | |
| 7 | f.F7OMT | 164 | 55.4 | GAGAGAAGTTACCAAGAATGGGC |
| 8 | r.F7OMT | 55.6 | CGAACCCTCCCTCCTCATA | |
| 9 | f.F4′OMT | 159 | 55.9 | GATAATGATGGCGGTGACGG |
| 10 | r.F4′OMT | 56.1 | GGCATTCAATTAAGGGTAGGCC | |
| 11 | f.F3H | 172 | 55.7 | TGAGAGAAGGTGAGAAGCCAA |
| 12 | r.F3H | 55.6 | GCAGGAGTTAGCCTTGTTTTCA | |
| 13 | f.F3′H | 167 | 56.2 | GACAGGAGGAAGTGGGGAT |
| 14 | r.F3′H | 56.8 | TCTGCCTTCTTGTCTCCACC | |
| 15 | f.F6H | 182 | 56 | CGGCAAGATTTACAAGGCGT |
| 16 | r.F6H | 56.4 | AAACTCCCTCATCACCTCCC | |
| 17 | f.RAS | 178 | 56.9 | CCGCAGCCTCTACTTCTACC |
| 18 | r.RAS | 56.8 | CTCGACGAACAACACTCCCT | |
| 19 | f.ACT | 175 | 55.4 | GGTGTGATGGTGGGTATGG |
| 20 | r.ACT | 55.5 | TCGTTGTAGAATGTGTGATGCC |