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. 2021 Oct 1;12(10):898. doi: 10.1038/s41419-021-04180-y

Fig. 3. LncRNA NLRP3 regulates the inflammatory response during ALI through NLRP3 inflammasomes.

Fig. 3

A qRT-PCR assay was used to analyse the mRNA expression of A lncRNA NLRP3, B NLRP3, C Caspase-1, D IL-18, E IL-1β, and F miR-138-5p in LPS -induced ALI. β-Actin was used as the reference gene. G, H ELISA was used to analyse the IL-1β and IL-18 levels in the culture supernatants. I, J Cell apoptosis was determined by Hoechst 33342 and PI dual staining assays (I) and counted (J). K Western blotting was used to analyse the protein expression of NLRP3 and caspase-1 after lncRNA NLRP3 overexpression in the cytoplasm. The data are presented as mean ± SE (n = 6). *P < 0.05; **P < 0.01; ***P < 0.001; NS, no statistically significant difference.