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. 2021 Jul 8;31(10):1106–1122. doi: 10.1038/s41422-021-00529-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — at-SNE visualization of ten lymphoid-related cell clusters on the scRNA-seq data of cells isolated from human fetal hematopoietic (liver), lymphoid (thymus and spleen) and non-lymphoid (intestine, skin and lung) tissues at 8,10 and 12 PCW. Color indicates the cell identity. b Dot plots show the featured genes expressed in different cell clusters. Colors represent the average gene expression levels, and size encodes the proportion of gene-expressing cells. c Dot plots show the expression level of the top 5 DEGs of each cell cluster. Colors represent the average expression and size encodes the proportion of gene-expressing cells. d Hierarchical clustering of lymphoid-related cell clusters based on Euclidean distance (left panel). The dynamic change of these populations during embryonic development (middle panel) and their tissue distribution (right panel) are shown in bar graph. Embryonic stages and tissues are indicated by colors. e Pie charts show the proportions of six progenitor clusters in each organ. The radii of sectors indicate proportions of each cell cluster.