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. 2021 Oct 1;12:5767. doi: 10.1038/s41467-021-26084-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematic representation of FL and deletion mutants of MeCP2. b Co-IP assays performed by anti-FLAG antibody in HEK293T cells expressing FLAG-tagged FL or deletion mutants of MeCP2 in the presence of nuclease and analyzed by western blotting with anti-GFP antibody. The average percentages of IP efficiency with standard deviations relative to the FL MeCP2 IP efficiency are shown below the gel (n = 3 biologically independent experiments). c IP assays performed by anti-FLAG antibody in HEK293T cells expressing RBFOX2 FL or its ΔCTD mutant in the presence of nuclease and analyzed by western blotting with anti-RBFOX2 antibody. d Representative results for GST pull-down analysis of the interactions between GST-tagged FL, ΔMBD, and ΔID of MeCP2 proteins and His-tagged RBFOX2. e Representative results for GST pull-down analysis of the interactions between GST-tagged MBD, ID, and MBD + ID of MeCP2 proteins and His-tagged RBFOX2. The average percentages of pulled down RBFOX2 relative to the input with standard deviations are shown below the gel in d, e (n = 3 biologically independent experiments). f Representative results for sedimentation profiles of the FL and mutant MeCP2 proteins in the HMW extract from HEK293T cells expressing FLAG-tagged MeCP2 proteins on 10–50% glycerol gradients. The FL and mutant MeCP2 proteins were detected by western blot analysis with anti-FLAG antibody. g Quantitation of the relative amount of MeCP2 proteins associated with Rbfox/LASR. The average percentages of MeCP2 proteins in fraction #8 to the sum of those in total fractions are normalized to that of FL MeCP2. Error bars represent standard deviation (n = 3 biologically independent experiments, *p < 0.05, ***p < 0.001, two-sided Student’s t test). Source data are provided as a Source data file.