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. 2021 Oct 1;12:5764. doi: 10.1038/s41467-021-26091-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a tSNE projection of immune cells from three ccRCC patients with normal PB cells (n = 13,433) and TI cells (n = 12,239). Treg population (blue) was isolated and separated as TI (orange) versus PB Treg cells (gray). b tSNE projection with the highlighted expression of Treg markers, FOXP3 and IL2RA (CD25). c Differential gene expression analysis using the log-fold change expression versus the difference in the percentage of cells expressing the gene comparing TI versus PB Treg cells (Δ Percentage Difference). Genes labeled have log-fold change > 1, Δ Percentage Difference > 20% and adjusted P-value from Wilcoxon rank sum test <0.05. d Top eight upregulated genes by log-fold change in TI Treg cells with adjusted P-value <0.05. e Top eight downregulated genes by log-fold change in TI Treg cells with adjusted P-value <0.05. d, e Wilcoxon rank sum test with p-values adjusted using the Bonferroni method. f Comparison of differential genes in TI Treg cells in ccRCC (orange) and HCC (green) compared to PB Treg cells. Significant genes were defined as log-fold change >1 or <−1 with adjusted P-values <0.05. g Violin plots showing relative mRNA level of Treg markers in PB (gray) and TI Treg cells in ccRCC (top) and HCC (bottom).