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. 2021 Oct 1;6:61. doi: 10.1038/s41536-021-00168-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a For all studies involving tendon tissue, it is important to define a single type of tendon and origin, and correctly define a control. b In case of cell seeding for tissue engineering, the cell type and origin of the cells must be evaluated carefully considering various extrinsic and intrinsic factors. If tenocytes are induced from stem cells, the phenotype should be evaluated by comparing it to the native tendon through ‘omics. In addition, if the objective of the study is to evaluate a construct, the effect of the construct should be studied initially, and then, additional variables of interest can be evaluated and compared to native tendon as a control. c For all studies, it is important to define the demographic factors to collect samples (sex, age, ethnicity, and health) according to the needs of the study and availability. d When possible, all medical information that may be provided should be updated to an online database. e The goal of the study defines the ‘omic techniques to use, yet we recommend using as many techniques as feasible. Created in Biorender.com.