Table 2.

Summary of significant unknown metabolites altered in the brain (cerebral cortex and brainstem) in ergotamine treated mice compared to control.

Group	TR (min)	Mass (m/z) [M + H]+	Tissue	ETHIGH versus ETVEH		ETLOW versus ETVEH		N-Way ANOVA
				Effect size	*	Effect size	*	Q-Value (BH adjusted p value)
1	8.27	623.3156	brainstem	1/70.85	↓	1/1.27	↓	0.014
2	2.48	86.09672	brainstem	1/1.00	↓	1/1.34	↓	0.039
3	4.96	199.0939	brainstem	9.81	↑	–	–	0.023
4	1.99	267.0585	brainstem	1.20	↑	1.43	↑	0.039
5	10.18	292.2992	brainstem	1/3.03	↓	1/1.41	↓	0.036
6	2.02	308.5818	brainstem	1.32	↑	1/1.52	↓	0.039
7	8.04	387.1794	brainstem	–	–	1/4.20	↓	3.23 × 10−6
8	12.5	387.1972	brainstem	1.50	↑	1/7.24	↓	0.006
9	2.39	408.1484	brainstem	1.13	↑	1/1.26	↓	0.039
10	8.04	425.1353	brainstem	–	–	1/4.59	↓	6.34 × 10−5
			cerebral cortex	–	–	1/4.76	–	1.11 × 10−5
11	2.26	437.1332	brainstem	1.48	↑	1/1.44	↓	0.023
12	2.39	559.1500	brainstem	1.31	↑	1/2.15	↓	0.025
13	5.39	910.849	brainstem	1/1.40	↑	2.93	↑	0.039
14	1.18	258.1096	cerebral cortex	1/1.33	↓	1.04	↑	2.51 × 10−4
15	1.28	269.0875	cerebral cortex	1.71	↑	1/1.59	↓	5.05 × 10−4
16	2.39	269.0876	cerebral cortex	1.70	↑	1/1.31	↓	6.84 × 10−4
17	2.39	291.0695	cerebral cortex	1.45	↑	1/1.21	↓	5.05 × 10−4
18	16.7	123.0553	cerebral cortex	1/4.53	↓	1.22	↑	7.11 × 10−4
19	2.39	137.0456	cerebral cortex	1.70	↑	1/1.28	↓	5.05 × 10−4
20	1.82	159.0276	cerebral cortex	1.82	↑	1/1.41	↓	8.93 × 10−4
21	2.55	162.0581	cerebral cortex	2.73	↑	1/1.27	↓	6.88 × 10−3
22	4.99	204.1050	cerebral cortex	3.07	↑	1/1.31	↓	5.05 × 10−4
23	1.35	218.1384	cerebral cortex	1/2.10	↓	1.15	↑	2.19 × 10−4
24	2.61	218.1384	cerebral cortex	1/1.86	↓	1.19	↑	5.05 × 10−4
25	7.06	246.1517	cerebral cortex	2.84	↑	1/1.25	↓	4.37 × 10−3
26	1.25	276.1185	cerebral cortex	2.01	↑	1/1.40	↓	6.87 × 10−3
27	1.28	307.0434	cerebral cortex	1.75	↑	1/1.44	↓	5.05 × 10−4
28	9.63	315.1949	cerebral cortex	7.93	↑	1/1.37	↓	8.93 × 10−4
29	2.39	353.0399	cerebral cortex	2.14	↑	1/1.52	↓	1.99 × 10−3
30	2.39	369.0123	cerebral cortex	1.67	↑	1/1.30	↓	4.37 × 10−3
31	2.45	387.9961	cerebral cortex	1.97	↑	1/1.47	↓	1.99 × 10−3
32	1.28	464.0806	cerebral cortex	1.68	↑	1/1.51	↓	9.31 × 10−3
33	2.45	502.0274	cerebral cortex	2.09	↑	1/1.40	↓	9.95 × 10−4
34	8.04	409.1614	brainstem	–	–	1/4.20	↓	5.34 × 10−7
			cerebral cortex	–	–	1/4.20	↓	1.51 × 10−9

*↑ = Up-regulated, ↓ = Down-regulated.