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. 2021 Oct 1;12:5779. doi: 10.1038/s41467-021-26049-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a 293T cells were transfected with increased Myc-SPOP WT in combination with Flag-Geminin and HA-Ub and harvested for IP and WB. b Control or SPOP knockout 293T cells were transfected with the indicated plasmids and harvested for IP and WB. c 293T cells transfected with plasmids for HA-tagged WT or mutated Ub in combination with other indicated constructs and harvested for IP and WB. d Mass spectrometry analysis reveals Geminin ubiquitination at lysine residues 27, 50, 100, and 127. e, f 293T cells were transfected with Flag-tagged WT or mutated Geminin in combination with other constructs and harvested for IP and WB. Source data are provided in this paper or Mendeley database (10.17632/8n7xt5rkhc.1). Similar results for (a), (b), (c), (e), and (f) panels were obtained in two independent experiments.