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. 2021 Oct 1;11:19550. doi: 10.1038/s41598-021-98911-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Time-lapse imaging showing evolution of GBM: HUVEC spheroid with HDF as the supporting cell at 1% O₂ over a period of 32 h. (A) Primary GBM (green) : HUVEC (red) spheroid. (B) NCH82 GBM (green) : HUVEC (red) spheroid. t = 0 h is taken at day 1 after seeding. HDF cells are non-fluorescent. The spheroids and single HDF cells are embedded in a 7.5 mg/mL fibrin gel. (C) Plot showing Pearson’s correlation coefficient as a function of culture time. Similar results were obtained in three independent experiments. (D) CD31 immunohistochemical staining (green) of primary GBM : HUVEC spheroid at day 3 after seeding. Right images are magnified views of the selected area (yellow box). The scale bars represent 100 $μ$ m. Subscript S denotes cells seeded in the same spheroid.