Skip to main content
. 2021 Oct 1;12:5772. doi: 10.1038/s41467-021-26061-w

Fig. 6. RNF213 counteracts CVB3 infection.

Fig. 6

a HeLa cells were infected with coxsackievirus (CV) B3 at MOI 0.01. Twenty four hours prior to infection, cells were transfected with a pool of siRNAs targeting RNF213 (siRNF213) or a pool of scrambled siRNAs (siScramble) as control. Twenty four hours post infection, the intracellular viral RNA load was determined by qRT-PCR (AVG ± SEM, n = 3 independent experiments, two-tailed unpaired t-test). b From the same experiment as in a, the intracellular viral protein load was determined by immunoblotting against VP1 and the intensity of the VP1 band is shown relative to the siScramble control (left panel, AVG ± SEM, n = 4 independent experiments, one-tailed t-test). A representative immunoblot for the quantification of VP1 is shown (right panel). c From the same experiment as in a, the viral titer was determined by counting PFUs after serial dilution (representative result from a single experiment, AVG ± SEM, n = 3 technical replicates, two-tailed unpaired t-test). Knockdown of RNF213 leads to a significant increase in CVB3 infection. In a–c asterisks indicate p values with *p < 0.05, **p < 0.01. Source data are provided as a Source Data file.