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. 2021 Oct 2;10:32. doi: 10.1186/s13619-021-00094-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Establishment of porcine and monkey colonic organoids. A, D Representative images of organoid growth of porcine colon (A) and monkey colon (D) in expansion medium from different passages. B, E Quantitation of the size of porcine colonic organoids (PCOs) (B) and monkey colonic organoids (MCOs) (E) in different passages. C, F Expression of cell marker genes was examined with q-PCR in primary colon tissue and organoids from different passages in pig (C) and monkey (F). Scale bars, 100 μm. *p < 0.05, ** p < 0.01, ***p < 0.001. Data are displayed as the mean ± SD by one-way ANOVA (B and E) and Student’s t-test (C and F). 30 organoids were calculated for the size in B and E. Three independent experiments were performed in C and F