Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 2;10:32. doi: 10.1186/s13619-021-00094-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Mature lineages of porcine colonic organoids are induced in differentiation medium. A Representative images of porcine colonic organoids grown in different differentiation media. Differentiation medium-1 (D-1): withdrawal of Wnt3a-CM and PGE2 from expansion medium with 2.5 μM CHIR-99021; Differentiation medium-2 (D-2): withdrawal of Wnt3a-CM, PGE2 and CHIR-99021 from expansion medium; Differentiation medium-3 (D-3): withdrawal of Wnt3a-CM, PGE2, CHIR-99021, nicotinamide and SB202190 from expansion medium. B Representative bright-field images and quantitation of the budding numbers of organoids in porcine colonic organoids cultured in expansion or differentiation medium for 7 days. White box depicts higher magnification below. C Expression of cell marker genes in proliferating (EM), differentiated (DM) porcine colonic organoids (passage 15) and tissue. D-G Fabp1 staining (D), ChgA staining (E), Alcian blue staining (F), Muc2 staining (G) in porcine colonic organoids cultured in expansion or differentiation medium for 7 days. Scale bars, 100 μm. *p < 0.05, ** p < 0.01, ***p < 0.001 analyzed by Student’s t-test. Data are shown as mean ± SD (n = 3 independent experiments)