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. 2021 Oct 2;10:32. doi: 10.1186/s13619-021-00094-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Mature lineages of monkey colonic organoids are induced in differentiation medium. A, B Representative images (A) and quantitation of organoid size (B) of monkey colonic organoids cultivated in expansion or differentiation medium for 7 days. White box depicts higher magnification below. C Expression of cell marker genes in proliferating (EM), differentiated (DM) monkey colonic organoids (passage 15) and tissue. D, E Fabp1 staining (D), Muc2 staining (E) of monkey colonic organoids cultured with expansion or differentiation medium for 7 days. Scale bars, 100 μm. *p < 0.05, ** p < 0.01, ***p < 0.001 analyzed by Student’s t-test. Data are shown as mean ± SD (n = 3 independent experiments)