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. Author manuscript; available in PMC: 2022 Mar 10.
Published in final edited form as: Cell Host Microbe. 2021 Feb 10;29(3):435–447.e9. doi: 10.1016/j.chom.2021.01.006

Fig. 1. HIV-infected macrophages evade efficient NK cell-mediated elimination.

Fig. 1.

(A) Schematic of the experimental process to coordinate maturation, activation, and infection of target cells with co-culture of autologous ex vivo NK cells. Details are described in the STAR Methods. (B-E) NK cell innate-based elimination assay. HIV-infected CD4+ T cells and macrophages were co-cultured with NK cells for 4 hours, overnight, or 48-hours at different effector-to-target (E:T) ratios, followed by measurement of Gag p24+ target cell elimination by flow cytometry. (B) Representative elimination assay plots. (C) Summary data from 4-hour co-cultures at an E:T of 2 from 9 independent experiments (n=15 biological replicates). (D) Summary data from overnight co-cultures at different E:T ratios. Shown are data from 5 independent experiments (n=8 biological replicates). (E) Summary data from 48-hour co-cultures at different E:T ratios. Shown are data from 4 independent experiments (n=6 biological replicates). Statistical analysis for (C-E): paired t tests, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, ns=not significant. See also Fig. S1.